• SOIL ECOLOGY. BIOGEOCHEMISTRY. Land use and global change.

     

     

    About

    I am a Research Associate at the USDA-ARS. I earned a Ph.D. in Forestry from the University of Montana in the spring of 2015. I also hold a M.S. in Geological Sciences and a B.A. in Environmental studies from the University of Colorado.

     

    As a microbial and ecosystem ecologist trained in soil science and biogeochemistry, I use molecular sequencing, soil functional assays, greenhouse experiments, and high-resolution biogeochemical measurements to understand the complex relationships between soil, microorganisms, and plants. I am specifically interested in the critical importance that soil biodiversity plays in regulating processes in natural and managed ecosystems.


    At the University of Minnesota, my Postdoctoral research examined how cropping system diversity and management techniques influence the composition and function of soil organisms, and in turn, how soil communities feedback to influence the quantity and quality of crop production. Now as a USDA-NIFA fellow, I'm working at the interface between native prairie and agro-ecosystems exploring the role of plant spatial and temporal diversity in structuring soil microbial communities and subsequent soil carbon cycling.

     

    During my doctoral work at the University of Montana, I investigated the process of microbial assembly and its implications for plant succession and biogeochemical cycling during early ecosystem development at retreating glaciers across the western hemisphere.

  • RESEARCH INTERESTS

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    Plant diversity impacts on soil carbon and microbial communities

    Understanding the factors that contribute to resilient, multi-functional plant microbiomes is an emerging goal of agroecological research. Plant diversity is perceived to be an important driver of soil microbial communities, but the specific mechanisms are relatively unknown. In agricultural and natural prairies, I am researching how soil organic matter quantity and quality contribute to the number of available niches for microorganisms, which may have important implications for microbial structure, microbial function, and microbe-microbe interactions.

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    Harnessing microbiomes to support resilient cropping systems

    In agricultural systems, soil microorganisms are important drivers nutrient availability, crop health, and productivity. At long-term agricultural research sites, I am researching how cropping system diversity and management strategies (nitrogen fertilization and cover cropping) contribute to soil microbial community assembly and function regionally across the upper Midwest.

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    Microbial community change during primary succession

    Following major disturbances that remove topsoil, microorganisms quickly colonize and begin the long process of soil formation by weathering minerals, decomposing organic matter, and mineralizing nutrients responsible for sustaining primary productivity. Using recently deglaciated landscapes as a model system, my research explores whether there are general patterns to microbial community succession across retreating glaciers. Additionally, my research explores the little known role of interactions between developing plant communities and soil biota during early succession.

  • Recent publications

    Please see my Google Scholar Profile for a full list of publications.

     

    Castle SC, Samac D, Sadowsky M, Rosen C, Gutknecht J, Kinkel LK (2019). Impacts of sampling design on estimates of microbial community diversity and composition in agricultural soils. Microbial Ecology. (DOI: 10.1007/s00248-019-01318-6)

     

    Castle SC, Song Z, Gohl DM, Gutknecht J, Rosen C, Sadowsky M, Samac D, Kinkel LK (2018). DNA template dilution impacts amplicon-sequencing based estimates of soil fungal diversity. Phytobiomes: PBIOMES-09.

     

    Darcy JL, Schmidt SK, Knelman JE, Nemergut DR, Cleveland CC, Castle SC. (2018) Phosphorus availability limits both visible and microscopic primary producers during the early stages of primary succession following glacial retreat. Science Advances.

     

    Cao Y, Miller S, Dornbusch M, Castle SC, Lenz P, Ferguson J, Sadowsky M, Nelson M, Klatt C, Samac D. (2018) Widespread occurrence of Sinorhizobium meliloti strains with a type IV secretion system. Symbiosis. https://doi.org/10.1007/s13199-018-0547-2

     

    Castle SC, Sullivan B, Knelman J, Hood E, Nemergut D, Schmidt S, Cleveland C. (2017) Nutrient limitation of soil microbial activity during the earliest stages of ecosystem development. Oecologia 185(3): 513-524.

     

    Castle SC, Nemergut DR, Grandy AS, Leff JW, Graham EB, Hood E, Schmidt SK, Wickings K, Cleveland CC. (2016) Biogeochemical drivers of microbial community convergence across actively retreating glaciers. Soil Biology and Biochemistry 101: 74-84.

     

    Castle SC, Lekberg Y, Affleck DLR, Cleveland C. (2016). Soil abiotic and biotic controls on plant performance during primary succession in a glacial landscape. Journal of Ecology doi: 10.1111/1365-2745.12615

     

    Graham EB, Knelman JE, Schindlbacher A, Siciliano S, Breulmann M, Yannarell A, Beman JM, Abell G, Philippot L ... Castle SC ... et al. Microbes as engines of function: does microbial community structure enhance predictions of ecosystem process rates? Frontiers in Microbiology, doi: 10.3389/ fmicb.2016.00214.

     

    Knelman JE, Schmidt SK, Lynch RC, Darcy JL, Castle SC, Cleveland CC, Nemergut DR (2014). Nutrient addition dramatically accelerates microbial community succession. PLOS ONE 9.

     

    Sullivan, BW, Alvarez-Clare SA, Castle SC, Porder S, Reed S, Schreeg L, Townsend AR, Cleveland CC (2013). Assessing nutrient limitation in complex forested ecosystems: alternatives to large-scale fertilization experiments. Ecology 95:668-681.

     

    Castle SC, Neff JC (2013). Controls on nutrient dynamics in high elevations: NUE across geological gradients in the San Juan Mountains of southern Colorado, USA. Oecologia 173: 1551-1561.

     

    Barger NN, Castle SC, Dean GN (2013). Denitrification from nitrogen-fixing biologically crusted soils in a cool desert environment, Southeast Utah, USA. Ecological Processes 2:16.

     

    Ross MR, Castle SC, Barger NN (2012). Fuels reduction effects on plant communities and soils in a Piñon-Juniper woodland. Journal of Arid Environments 79:84-92.

     

    Castle SC, Morrison CD, Barger NN (2011). Extraction of chla from biological soil crusts: A comparison of solvents for spectrophotometric determination. Soil Biology and Biochemistry 43:853-856.

     

    Castle SC, Neff JC (2009). Plant response to nutrient availability across bedrock geologies. Ecosystems 12:101-113.

     

  • Useful links

    Data and visualization

    Microbiome data analysis

    Microbiome Resources

    Science adjacent work

  • CONTACT

    Sarah C. Castle
    USDA-ARS

    University of Minnesota
    Department of Soil, Water, and Climate
    439 Borlaug Hall, 1991 Upper Buford Circle
    Saint Paul, MN 55108

     

    Office: 429 Soil Sciences Building
    sccastle@umn.edu